Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel
Identifieur interne : 000238 ( France/Analysis ); précédent : 000237; suivant : 000239Enhancement of somatic embryogenesis frequency by gibberellic acid in fennel
Auteurs : Gérard Hunault [France] ; Abdelaziz Maatar [France]Source :
- Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1995-05-01.
Abstract
Abstract: The effect of GA3 on somatic embryogenesis from petiole fragments excised from micropropagated fennel plantlets was studied. Explants were maintained for 4 weeks on an induction medium containing, 2,4-d and kinetin and were then transferred to a medium devoid of these growth regulators to allow embryo development. The addition of autoclaved or filter-sterilized GA3 to the induction medium or to the embryo development medium increased the number of embryogenic explants. No positive effect was observed when GA3 was added to the micropropagation medium of the mother plantlets. Gibberellic acid also counteracted the inhibiting effect of continuous light on the number of embryogenic explants. Moreover, the embryogenic frequency of petiole explants from several genotypes previously considered as poorly reacting was highly enhanced by GA3.
Url:
DOI: 10.1007/BF00051587
Affiliations:
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ISTEX:7FF25D57EBB76A7A2093B097395F9869A255895CLe document en format XML
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